Introduction
THC acetate has twice the potency of THC. On the Adams scale, THC 7.3, while its acetate = 14.6. Furthermore, there is a 25% increase in weight after adding the acetate structure. The effect of the acetate is more spiritual and psychedelic than that of the ordinary product. The most unique property of this material is that there is a delay of about thirty minutes before its effects are felt.
This procedure was provided many times with varied efficiency. This variability was caused by and directly proportional to the amount of essential oil in the reaction. The more oil, the less efficient the reaction. The maximum amount of oil I use is 20 g.
Safety note: This is where things can hit the fan! You should have proper PPE including a face shield, chemical resistant apron, and chemical gloves. This again should be done in a fume hood.
This procedure was provided many times with varied efficiency. This variability was caused by and directly proportional to the amount of essential oil in the reaction. The more oil, the less efficient the reaction. The maximum amount of oil I use is 20 g.
Safety note: This is where things can hit the fan! You should have proper PPE including a face shield, chemical resistant apron, and chemical gloves. This again should be done in a fume hood.
Equipment and glassware:
- Flat bottom boiling flask;
- 5 L bucket of ice water;
- Aquarium water pump;
- Pasteur pipette's;
- Laboratory scale (1 — 500 g is suitable);
- 5 L x2; 1 L x2; 500 ml x2; 100 ml x2 Beakers;
- 1 L and 100 ml Measuring cylinders;
- Filter paper;
- Conventional funnel;
- Separatory funnel;
- pH indicator papers (optional);
- Boiling stones;
- Water-jet aspirator (optional);
- Rotary evaporator (optional);
- Glass rod;
- Water/oil bath;
Reagents:
- Dry ice in a foam cooler
- 2 L 95% Ethyl Alcohol;
- 2 L Denatured alcohol;
- Hexane (HPLC grade);
- 98% Sulphuric acid;
- ~7 L Distilled water;
- ~200 g Sodium chloride (NaCl);
- ~100 g Baking soda (NaHCO3);
Boiling Point: 172-174 °C/0.08 Torr; 421.77 °C at 760 mm Hg ;
Melting Point: 160.18 °C;
Molecular Weight: 356.50 g/mol;
Density: 1.0±0.1 g/ml (20 °C).
CAS Number: 23132-17-4.
Melting Point: 160.18 °C;
Molecular Weight: 356.50 g/mol;
Density: 1.0±0.1 g/ml (20 °C).
CAS Number: 23132-17-4.
Procedures:
1. Dry your THC in an oven at 105 ℃ until thoroughly dry and free of any alcohols;
2. Dissolve your extract into an alkane solvent, such as hexane (HPLC grade);
3. In a flat bottom boiling flask, combine extract in a 1:2.5 w/v ratio of oil to acetic anhydride. (1 g + 2.5 mL);
4. Place it in an ice and water bath and add 2.5 drops/g oil of 98% H2SO4 slowly down the side of the glass, swirling gently after every couple of drops;
5. Once the solution has cooled, place a stir bar in carefully to avoid splashes;
6. Put on a stir plate and begin stirring vigorously;
7. Place a reflux condenser onto the top;
2. Dissolve your extract into an alkane solvent, such as hexane (HPLC grade);
3. In a flat bottom boiling flask, combine extract in a 1:2.5 w/v ratio of oil to acetic anhydride. (1 g + 2.5 mL);
4. Place it in an ice and water bath and add 2.5 drops/g oil of 98% H2SO4 slowly down the side of the glass, swirling gently after every couple of drops;
5. Once the solution has cooled, place a stir bar in carefully to avoid splashes;
6. Put on a stir plate and begin stirring vigorously;
7. Place a reflux condenser onto the top;
8. Begin heating until the vapor is being condensed at the second bulb. This takes a bit of adjusting to get to the perfect plate temperature versus condenser water flow;
9. Start a timer for 60 min for up to 10 g and add 60 min for up to an additional 10 g of oil;
10. Turn off the hot plate but continue to stir;
11. When cooled slightly, add 1 mL (per 1 g oil) of distilled water drop wise against the glass;
12. Vapors containing acetic acid will escape from the top. This is normal. DO NOT INHALE VAPORS!
13. Pour liquid into a separatory funnel, wash flask clean with hexane and pour into funnel. Wash flask of anything left with 95% non-denatured alcohol, pour into funnel;
14. Prepare ~7 L of distilled water with dissolved 200 g NaCl;
9. Start a timer for 60 min for up to 10 g and add 60 min for up to an additional 10 g of oil;
10. Turn off the hot plate but continue to stir;
11. When cooled slightly, add 1 mL (per 1 g oil) of distilled water drop wise against the glass;
12. Vapors containing acetic acid will escape from the top. This is normal. DO NOT INHALE VAPORS!
13. Pour liquid into a separatory funnel, wash flask clean with hexane and pour into funnel. Wash flask of anything left with 95% non-denatured alcohol, pour into funnel;
14. Prepare ~7 L of distilled water with dissolved 200 g NaCl;
15. Once the water can be distinguished from the hexane, begin draining the water;
16. When you are close to the emulsion layer, stop and add salt water until the funnel is almost full;
17. Cap and mix by inverting several times, uncap or the pressure will shoot the lid off;
18. You should have a lower water layer an emulsion layer and an upper organic layer;
17. Cap and mix by inverting several times, uncap or the pressure will shoot the lid off;
18. You should have a lower water layer an emulsion layer and an upper organic layer;
19. Continue rinsing with NaCl water solution until it runs out;
20. Drain the organic layer into a clean beaker and place into an oil bath 120 ºС until dry or use vacuum rotary evaporator with mild water bath heating (the best way);
21. Test a small drop of acetate on a watch glass with some baking soda dissolved in water. If it fizzes, re-suspend acetate in hexane and wash with baking soda water solution until it stops bubbling. You can use pH indicator paper for determining neutral pH (~7-7.5) of the THC-O-Acetate solution. Re-separate water from organic in funnel;
22. Dry at 120 ºС or use vacuum rotary evaporator with mild water bath heating;
23. Re-dissolve in 95% EtOH and place beaker into a shallow, denatured alcohol and dry ice bath for at least 5 min;
24. Pour liquid through a coffee filter and wash filter by a small amount of 95% EtOH;
25. Discard filter and dry the acetate by boiling off the alcohol. Using vacuum rotary evaporator, preferably;
26. Wash and dry 2 more times with ethanol (steps 23-25).
Enjoy!
20. Drain the organic layer into a clean beaker and place into an oil bath 120 ºС until dry or use vacuum rotary evaporator with mild water bath heating (the best way);
21. Test a small drop of acetate on a watch glass with some baking soda dissolved in water. If it fizzes, re-suspend acetate in hexane and wash with baking soda water solution until it stops bubbling. You can use pH indicator paper for determining neutral pH (~7-7.5) of the THC-O-Acetate solution. Re-separate water from organic in funnel;
22. Dry at 120 ºС or use vacuum rotary evaporator with mild water bath heating;
23. Re-dissolve in 95% EtOH and place beaker into a shallow, denatured alcohol and dry ice bath for at least 5 min;
24. Pour liquid through a coffee filter and wash filter by a small amount of 95% EtOH;
25. Discard filter and dry the acetate by boiling off the alcohol. Using vacuum rotary evaporator, preferably;
26. Wash and dry 2 more times with ethanol (steps 23-25).
Enjoy!
Last edited:
